Dynamic study of oral Candida infection and immune status in HIV infected patients during HAART.

OBJECTIVETo dynamically monitor oral Candida infection, CD4+ T lymphocyte counts, CD8+ T lymphocyte counts and CD4/CD8 ratios in HIV/AIDS patients during their first year of HAART and to preliminarily explore the relationships between them.DESIGNForty-six patients with HIV/AIDS receiving HAART at the Infection Department of Chongqing Red Cross Hospital were followed for one year.

At baseline and 3, 6, and 12 months after HAART initiation, oral rinses were collected and cultured to identify Candida species. Further, blood samples were collected to detect CD4+ T lymphocyte counts, CD8+ T lymphocyte counts and CD4/CD8 ratios.

TBS buffer (Tris-Buffered Saline), 10x Solution

A0027 4L
EUR 93.93

TBS buffer (Tris-Buffered Saline), 10x Solution

UA00279 500ml
EUR 58.48

Tris Buffered Saline (TBS) 10x pH 8.0, 1000 ml

12-9134-10 1000 ml
EUR 459

TBS

I004 1000 ml
EUR 219
Description: TBS by Cygnus Technologies is available in Europe via Gentaur.

T-Pro Washing buffer in TBS and Tween-20 (10X)

JB09-I004 500ml/BT
EUR 144

T-Pro Tris-Buffered Saline (TBS, 10X) for western blot washing

JB09-I002 500ml/BT
EUR 135

Tris Buffered Saline (TBS, pH 7.4) concentrate (10X, 100 mM Tris/150mM NaCl final)

80082 1 L
EUR 225

Tris Buffered Saline (TBS, pH 7.4) concentrate (10X, 50 mM Tris/150mM NaCl final)

80083 1 L
EUR 225

EZBlock? (TBS) Blocking Buffer

2117-1000
EUR 212

EZBlock? (TBS) Blocking Buffer

2117-200
EUR 125

TBS Wash Concentrate, 20X

F004 50 ml
EUR 180
Description: TBS Wash Concentrate, 20X by Cygnus Technologies is available in Europe via Gentaur.

TBS Wash Concentrate, 20X

F004-1000 1000 ml
EUR 403
Description: TBS Wash Concentrate, 20X by Cygnus Technologies is available in Europe via Gentaur.

TBS Blocking Buffer Pack

AR0143 200mL/pack
EUR 65

TBS Wash Buffer Pack

AR0144 500mL/pack
EUR 60

SkipDewax, 10X

T213 50ml
EUR 297

PBS, 10X

UPD8117 500ml
EUR 65.66

THROMBOSTEP

TBS-25 25 test
EUR 778

THROMBOSTEP

TBS-50 50 test
EUR 986

EZBlock? T20 (TBS) Blocking Buffer

2140-1000
EUR 218

EZBlock? T20 (TBS) Blocking Buffer

2140-200
EUR 120

Tris Buffered Saline (TBS) Powder

AR0031 2000mL/pack
EUR 53

TBS Buffer, 20X pH 7.4

T8054-050 500ml
EUR 86

TBS Buffer, 20X pH 7.4

T8054-101 1L
EUR 99

TBS Buffer, 20X pH 7.6

T8055-050 500ml
EUR 86

TBS Buffer, 20X pH 7.6

T8055-101 1L
EUR 99

TBS blocking buffer with BSA

SD8113 1pk, 1L
EUR 83.93

PBS (10X Concentrate)

85R-125 250 ml
EUR 235
Description: Phosphate Buffered Saline (PBS) 10X concentrate

TissueDigest Diluent, 10X

T102 10ml
EUR 94

10x Taq Buffer

BA00151 1.8ml
EUR 55
Description: High purity buffer for various PCR applications.

10X TAE Buffer

T8048-101 1L
EUR 89

10x Proteinase K

TG4039 0.5ml
EUR 134

NH4 Buffer, 10x

BIO-37025 3 x 1.2ml Ask for price

Trypsin, 2.5%(10X)

CA019-010 100ml
EUR 80

Collagenase/Hyaluronidase (10X)

CA094-002 20ml
EUR 340

10X Loading Buffer

L0503-005 5X1ml
EUR 77

10X Loading Buffer

L0504-005 5X1ml
EUR 77

10X MOPS Buffer

M1057-050 500ml
EUR 161

10X MOPS Buffer

M1057-100 2X500ml
EUR 219

450ML PBS 10X

NAT1006 450ML
EUR 80

4L PBS 10X

NAT1008 4L
EUR 199

1L TBE 10X

NAT1196 1L
EUR 82

4L TBE 10X

NAT1198 4L
EUR 121

Casein-based TBS Blocking Buffer Pack

AR0183 500mL/Pack
EUR 70

ChonBlock Western Blot Buffer 1 (TBS)

9069T 10 ml
EUR 193.55
Description: ChonBlock Western Blot Buffer 1 (TBS)

TBST (TBS-Tween 20), 10XpH 7.6

UA0046 500ml
EUR 69.93

TBS with non fat milk premix

SD8104 1pk
EUR 63.49

Binding/Coating Buffer (10X)

85R-124 250 ml
EUR 216
Description: ELISA buffer for optimal coating and binding of antibodies and antigens

10X KRB-IBMX Buffer

122937 1 Vial
EUR 77

Blocking Buffer, 10X, 250ML

X109-250ML 250ML
EUR 359

Blocking Buffer, 10X, 25ML

X109-25ML 25ML
EUR 122

Alkaline Phosphate Buffer, 10x

K2191050-6 50 ml
EUR 137
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

10x Taq Buffer (MgCl2)

PCRB16 4x1.5ml, 6ml
EUR 58.7

10X TAE buffer solution

A00239 500ml
EUR 56.36

RBC Lysis Solution 10X

abx290033-50ml 50 ml
EUR 230

Tissue Pre-Conditioner, 10X

T201 50ml
EUR 297

SkipDewax Trial Size, 10X

T212 10ml
EUR 123

10x TAE Buffer Solution

BA01801 6x100ml
EUR 97
Description: High purity buffer for various PCR applications.

10x PBS Buffer Solution

BA01802 6x100ml
EUR 97
Description: High purity buffer for various PCR applications.

10x TBE Buffer Solution

BA01804 6x100ml
EUR 97
Description: High purity buffer for various PCR applications.

TBST(10X), pH 7.4

T8056-100 1L
EUR 115

10x Gel Loading Buffer

TG4038 1ml
EUR 156

Trypsin-EDTA(10X), 0.5%

CA015-010 100ml
EUR 88

Trypsin-EDTA(10X), 0.5%

CA015-100 10x100ml
EUR 286

Trypsin-EDTA(10X), 0.5%

CA015-200 20x100ml
EUR 482

Trypsin-EDTA(10X), 0.05%

CA022-010 100ml
EUR 84

25ML 10X CE Buffer

NAT1206 25ML
EUR 115

100ML 10X CE Buffer

NAT1208 100ML
EUR 164

RBC Lysis Solution 10X

RBC10X-50ML 50 ML
EUR 108.5

TBS buffer (Tris-Buffered Saline), 20x Solution

UA0045 500ml
EUR 66.97

T-Pro Fast Blocking Buffer (in TBS)

JK92-W002 500ml/BT
EUR 187

10x PBST Solution (10X Phosphate Buffered Saline, 0.5% Tween-20), PH 7.4

UPD8119 500ml
EUR 67.4

EIA Wash Buffer 10x concentrate

1003-1 1 Liter
EUR 231

10X Annexin V Binding Buffer

1006-100
EUR 196

Glutathione Reductase Conc., 10x, 1ML

X130-1ML 1ML
EUR 123

Annexin V Binding Buffer (10x)

EXB0019 N/A
EUR 86

SuperBlot Western Transfer buffer 10X

DG-WEST 500ml
EUR 113.8

10x Taq Buffer (NH4)2SO4

PCRB55 4x1.5ml, 6ml
EUR 58.7

1 L TAE BUFFER, 10X

46-010-CM 1L/pk
EUR 105
Description: Media Catalog; Classical Media

PBST buffer 10x Strength Solution

PW004 500ml
EUR 67.4

TBST buffer 10x Strength Solution

PW009 500ml
EUR 65.66

10x PBS Ready Mixed Powder

BA01701 1L
EUR 68
Description: High purity buffer for various PCR applications.

10x TBE Ready Mixed Powder

BA01704 1L
EUR 85
Description: High purity buffer for various PCR applications.

10x Tris-Glycine Buffer Solution

BA01806 6x100ml
EUR 97
Description: High purity buffer for various PCR applications.

10X Tris-Glycine SDS buffer

T8053-050 500ml
EUR 85

10X Tris-Glycine SDS buffer

T8053-100 2X500ml
EUR 111

10X Tris-Glycine SDS buffer

T8053-101 1L
EUR 99

10X Tris-Glycine SDS buffer

T8053-104 4 L
EUR 153

10X Tris-Glycine SDS buffer

T8053-200 4X500ml
EUR 136

10X Tris-Glycine SDS buffer

T8053-201 2X1L
EUR 135

10X Tris-Glycine SDS buffer

T8053-401 4X1L
EUR 172

Topo I Assay Buffer 10x

TG4010 1ml
EUR 145

West-Ezier Washing Buffer(10X)

W3873-050 500ml
EUR 118

10X Fish Gelatin Blocking Agent

22010 100ML
EUR 112
Description: Minimum order quantity: 1 unit of 100ML

10X Phosphate-Buffered Saline (PBS)

22020 4L
EUR 200
Description: Minimum order quantity: 1 unit of 4L

Aniline, 10X in acetate buffer

91057 10ML
EUR 111
Description: Minimum order quantity: 1 unit of 10ML

Citrate Buffer (10x) pH 6.0

CBB125 125 ml
EUR 66

Citrate Buffer (10x) pH 6.0

CBB500 500 ml
EUR 80

Citrate Buffer (10x) pH 6.0

CBB999 1000 ml
EUR 98

Colloidal Gold Conjugate Diluent (10X)

CG-CD10 10 ml
EUR 225

Colloidal Gold Running Buffer (10X)

CG-RB10 10 ml
EUR 225

Citrate Plus (10x) HIER Solution

CPL500 500 ml
EUR 138

Citrate Plus (10x) HIER Solution

CPL999 1000 ml
EUR 205

Dilution buffer R (10x); ELISPOT

CT348 10 ml
EUR 78

Dilution buffer T (10x); ELISPOT

CT358 8 ml
EUR 78

Blocking stock solution (10x); ELISPOT

CT363 10 ml
EUR 78

10X PBS Buffer , pH 7.4

P2100-050 500ml
EUR 88

10X PBS Buffer , pH 7.4

P2100-100 1L
EUR 91

Xpert Native Transfer Buffer(10X)

P9001-050 500ml
EUR 93

Xpert Native Transfer Buffer(10X)

P9001-100 1L
EUR 138

10x PCR Buffer without MgCl2

ABP-PP-ERBF001 1x1ml Ask for price

10x PCR Buffer without MgCl2

ABP-PP-ERBF002 2x1ml Ask for price

10x PCR Buffer without MgCl2

ABP-PP-ERBF005 5x1ml Ask for price

10x PCR Buffer with MgCl2

ABP-PP-ERBFM01 1x1ml Ask for price

10x PCR Buffer with MgCl2

ABP-PP-ERBFM02 2x1ml Ask for price

10x PCR Buffer with MgCl2

ABP-PP-ERBFM05 5x1ml Ask for price

10X PBS MaxTag Histo (OKRA00043)

OKRA00043 100 ml
EUR 140
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

ChonBlock Western Blot Buffer 2 for Goat (TBS)

90691T 10 ml
EUR 121.05
Description: ChonBlock Western Blot Buffer 2 for Goat (TBS)

ChonBlock Western Blot Buffer 2 for Rabbit (TBS)

90692T 10 ml
EUR 121.05
Description: ChonBlock Western Blot Buffer 2 for Rabbit (TBS)

Tris Buffered Saline (TBS) pH 7.6, 500 ml

09-7500-100 500 ml
EUR 205

Tris Buffered Saline (TBS) pH 7.6, 500 ml

09-7500-30 500 ml
EUR 128

Tris Buffered Saline (TBS) pH 8.0, 1000 ml

12-9133-10 1000 ml
EUR 99

T-Pro Protein Free Blocking Buffer (in TBS)

JK92-W004 500ml/BT
EUR 187

10X Red Blood Cell Lysis Buffer

5831-100
EUR 120

TG Buffer (TRIS-GLYCINE) 10X Solution

A0028 4L
EUR 94.8

DPBS 10X (WITHOUT CALCIUM AND MAGNESIUM)

20-031-CV 500 mL/pk
EUR 80
Description: Media Catalog; Buffered Salt-Salution

RESULTSThe prevalence of OC decreased after HAART initiation (P < 0.05), and Candida albicans was the dominant species isolated from the oral cavity (66/93). At 3, 6 and 12 months after HAART initiation, CD4+ T lymphocyte counts were 327.91 ± 138.82, 329.65 ± 142.66 and 319.98 ± 97.90 cells/mm3, respectively, which were significantly higher than the level at baseline (263.39 ± 126.01 cells/mm3) (P = 0.016, P = 0.014, and P = 0.035, respectively).

During the first year of HAART, CD4/CD8 ratios increased gradually, and CD8+ T lymphocyte counts decreased continually. OC was associated with low CD4+ T lymphocyte counts and a low CD4/CD8 ratio. CD4+ T lymphocyte counts <200 cells/mm3 and Candida load ≥300 CFU/mL were risk factors for OC (P < 0.05), and oral Candida load was negatively correlated with CD4+ T lymphocyte counts and the CD4/CD8 ratio.OC might be a useful marker for the evaluation of immune status in patients with HIV/AIDS.